siRNA Screening of a Targeted Library of DNA Repair Factors in HIV Infection Reveals a Role for Base Excision Repair in HIV Integration
Figure 3
MEFs lacking BER genes have decreased transduction by HIV-based retroviral vectors.
MEFs derived from mice with deletions of the Neil1, Myh, Ogg1, Polβ, or a double deletion of Myh and Ogg1 genes. Cells were transduced with an HIV retroviral vector expressing GFP and analyzed at 72 hours post-infection by flow cytometry for GFP expression indicating successful infection. The percentage of GFP positive cells is shown relative to wild type MEFs from matched littermates. (A) Wild type, Neil1−/−, Myh−/−, Ogg1−/−, Myh−/− Ogg1−/−, and Polβ −/− (PolB−/−) cell lines, (B) Wild type (Neil1+/+), heterozygous (Neil1+/−), and Neil1−/− cell lines. Infections were performed at two MOI in duplicate at least three times. Error bars indicate the standard deviation after normalization.