Characterization of Bioactive Recombinant Human Lysozyme Expressed in Milk of Cloned Transgenic Cattle
Figure 4
Thermostability of purified rHLZ and HLZ at 60°C, 80°C, and 100°C.
The lysozyme activity assay was carried out using the turbidimetric method with a spectrophotometer at A450. Lytic activity was measured against Micrococcus lysodeikticus in 66 mM potassium phosphate buffer, pH 6.24, at 25°C after incubation for different times at 60°C (A), 80°C (B), and 100°C (C). Lysozyme activity measured at 25°C represented 100% activity. rHLZ, purified rHLZ; HLZ, commercial HLZ. The experiment for each group was repeated at least three times, and the results represent mean ± S.D.