Contribution of CgPDR1-Regulated Genes in Enhanced Virulence of Azole-Resistant Candida glabrata
Figure 7
Overexpression of CgCDR1 and PUP1 in a CgPDR1-independent manner.
Panel A: TDH3-dependent expression of CgCDR1. Panel B: TDH3-dependent expression of PUP1. Quantification was performed by qRT-PCR. The values are averages of three separate experiments and represent the increase in gene expression relative to SFY196 (set at 1.00). Strains derived from DSY562 are represented by black bars and the indicated names correspond to the following strains: PDR1: SFY196, pdr1Δ: SFY198, pdr1Δ+TDH3p-CDR1: SFY200, pdr1Δ+TDH3p-PUP1: SFY202. Strains derived from DSY565 are represented by white bars and the indicated names correspond to the following strains: PDR1L280F: SFY197, pdr1Δ: SFY199, pdr1Δ+TDH3p-CDR1: SFY201, pdr1Δ+TDH3p-PUP1: SFY203.