Nuclear Receptor Rev-erb Alpha (Nr1d1) Functions in Concert with Nr2e3 to Regulate Transcriptional Networks in the Retina
Figure 3
Injection with shNr1d1 results in pan-retinal spotting and reduced response to light in scotopic (dark-adapted) and photopic (light-adapted) experiments.
Mice were injected at postnatal day (P) 0.5 and aged to P30.5 for clinical and functional phenotyping. I: shNr1d1 (A) produced pan-retinal spotting visible on the fundus. Injection with shCryaa (B) resulted in lens opacity. Vector only injections (C) resulted in no visible fundus phenotype. II: Graphic representation of scotopic electroretinogram testing the function of rod cells, and photopic electroretinogram testing the function of cone cells, reveled that shNr1d1 reduced the response to light by 50% compared with controls. Responses from photoreceptor cells and second order neurons were affected. shNr1d1 n = 3, B6 control n = 5. Statistical significance of a two-tailed unpaired t-test are shown as: *: p<0.05; **: p<0.01.