Evaluation of Magnetic Micro- and Nanoparticle Toxicity to Ocular Tissues
Figure 5
Measure of astrocyte activation using GFAP staining.
Example GFAP immunofluorescence images for the PBS- (control), 50 nm- and 4 µm-intravitreally injected animals at 1 hour, 1 week and 5 months as marked. AC-injected animals are also shown for the 5 month time point. Astrocyte activation is seen along the ganglion cell layer (red) with nuclear counterstaining with DAPI (blue) highlighting the retinal layers. Astrocyte activation was similar among PBS- and magnetic particle-injected animals at all three time points for both types of injections. For IVT-injected animals, GFAP staining was seen primarily at the site of injection along the ganglion cell layer. Scale bar, 50 µm.