Identification of Baicalin as an Immunoregulatory Compound by Controlling TH17 Cell Differentiation
Figure 5
Baicalin inhibits IL-17+ lymphocyte infiltration into kidney.
(A) IL-17 immunohistochemical staining in kidneys from MRL/lpr mice treated with Baicalin or vehicle for 9 weeks, arrow shows the typical IL-17+ lymphocytes. Scale bar, 100 µm. Five independent microscopic fields were selected randomly for each sample to ensure representativeness and homogeneity. Right panel shows the IL-17+ cells counted under ×100 magnification. (B) CCL20 mRNA expression in kidney. (C) CD4+CD25− T cells from B6 mice were cultured under TH17 conditions with or without Baicalin for 3 days, CCR6 mRNA expression in TH17 cells was examined by RT-PCR. These experiments were performed three times with similar results.