C-Type Lectin in Chlamys farreri (CfLec-1) Mediating Immune Recognition and Opsonization
Figure 5
rCfLec-1 promotes encapsulation by scallop hemocytes.
Nickel agarose beads coated with rCfLec-1, or rTrx were incubated with scallop hemocytes. Encapsulation of the protein-coated beads was observed by microscopy after 6 and 24 h incubation. rCfLec-1-coated agarose beads were pre-incubated with antibody of rCfLec-1, and encapsulation of the antibody-bound beads was performed as above and observed. (A) Protein-coated beads were encapsulated by scallop hemocytes, and hemocyte encapsulation of the lectin-coated beads was block by antiserum specific for rCfLec-1, bar = 50 µm. (B) The numbers of encapsulated at 6 h and 24 h beads. The columns represent the mean of three individual counts ± S. E. M.