Expression Profiling of Major Histocompatibility and Natural Killer Complex Genes Reveals Candidates for Controlling Risk of Graft versus Host Disease
Figure 8
Analysis of MHC and NKC gene regulation in GVHD skin lesions from transplanted animals.
BN (RT1n) rats were transplanted with bone marrow of PVG (RT1c) rats. Rats that developed acute GVHD were scarified and skin lesions with signs of GVHD were obtained for RNA preparation and histology. The expression of 7 MHC and 3 NKC was analyzed by qRT-PCR using the B2m gene as reference. The relative changes of gene expression levels were calculated [24]. The means plus SEM are shown for skin lesion with grade I and grade II GVHD. A value >1 indicates an up-regulation of gene expression in the allogeneic samples. The control samples were freshly frozen skin samples from healthy BN rats (n = 7).