Profiling the HER3/PI3K Pathway in Breast Tumors Using Proximity-Directed Assays Identifies Correlations between Protein Complexes and Phosphoproteins
Figure 4
Effects of trastuzumab, 2C4 and lapatinib on molecular markers under HRG-stimulated conditions.
T47D cells were exposed to trastuzumab, 2C4, lapatinib or left untreated prior to treatment with heregulin. VeraTag assays to measure (A) HER23D, (B) HER3P, (C) p/t-Akt and (D) HER3PI3K were performed on FFPE sections prepared from these cells. The normalized RPA values are plotted on the y-axis. The treatments are indicated on the x-axis.