A Novel Role for ATM in Regulating Proteasome-Mediated Protein Degradation through Suppression of the ISG15 Conjugation Pathway
Figure 3
ATM negatively regulates the ISG15 pathway.
Extracts of FT169A (A-T) and FT169A (ATM+) cells were analyzed by 5% (top panel) or 15% (middle panel) SDS-PAGE, followed by immunoblotting using either anti-ATM (top panel) or anti-ISG15 antibody (middle panel). The same membrane shown in the second panel was stripped and re-probed with anti-tubulin antibody to assure equal protein loading. Average band intensity of the free ISG15 protein (error bar represents SEM) from three independent experiments was quantified using Kodak Image Station 2000R and the results are shown in the bar graph.