The Development of Metabolomic Sampling Procedures for Pichia pastoris, and Baseline Metabolome Data
Figure 1
Comparison of metabolite extraction methods.
A) Metabolite concentrations extracted from P. pastoris either with buffered boiling ethanol (EtOH) or using a freeze thaw methodology with aqueous methanol (MeOH). Concentrations were determined from 1H NMR spectra of the dried extracts using the Chenomx software suite. Data was normalised by either the cellular debris protein concentration (left: nM/mg cell debris protein) or by the probabilistic quotient method (right: nM) [19], and log transformed. MO = 3-Methyl-2-oxovalerate, GPC = Glycero-3-phosphocholine. B) Correlation of metabolite concentrations extracted from P. pastoris with either buffered boiling ethanol or methanol; C) Relative standard deviation of replicate concentration measurements of metabolites extracted from P. pastoris with either buffered boiling ethanol or methanol.