Point Mutations in Aβ Result in the Formation of Distinct Polymorphic Aggregates in the Presence of Lipid Bilayers
Figure 8
Representative in situ AFM images of Flemish Aβ (1–40) aggregate formation on supported TBLE lipid bilayers.
(A) Within 3–5 hours after the injection of Flemish Aβ (1–40) into the fluid cell, oligomeric aggregates appeared on the bilayer surface. (B) Often small defects in the lipid bilayer that did not appear to be associated with aggregates would develop. There were also many large amorphous aggregates on the bilayer. (C) After ∼10–12 hours of exposure to Flemish Aβ (1–40), a few small patches of increased bilayer roughness associated with a high density of very short putative fibrillar aggregates developed. (D) Not all fibrils were associated with bilayer disruption.