Identification and Validation of Novel Cerebrospinal Fluid Biomarkers for Staging Early Alzheimer's Disease
Figure 2
Unsupervised clustering of CSF samples by 2D-DIGE data from the 119 statistically significant gel features.
(Student's t-test, α = 0.05, present in >50% of images). Five gels containing hemoglobin (n = 10 samples) were excluded. Columns represent samples; rows, numbered 1 through 119 from top to bottom, represent gel features depicted in Figure 1. Gel feature intensity is encoded colorimetrically from red (low intensity) to green (high intensity); white indicates absent data. CDR status of individuals at time of CSF collection is encoded below by small green (CDR 0) and red (CDR 1) ovals; CDR 0 and CDR 1 clusters are indicated below by green and red bars, respectively. APOE-ε4 allele status of individuals and groups, alike, is indicated by black (possessing ApoE4 protein, or one or two APOE-ε4 alleles) or blue (possessing no ApoE4 protein, or no APOE-ε4 alleles) bars. Rows representing gel features containing ApoE protein are indicated along the lower right border.