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Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products

Figure 5

Quality scores for all overlapped 120 bp composite reads.

The scores a log-odds score of the likelihood of error in the base call, higher scores represent lower likelihoods of error [40]. They are expected to decrease with distance from the left or right sequencing primer, and to be highest in the region of perfect overlap because scores are additive.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0015406.g005