Vaccinia-Related Kinase 1 Is Required for the Maintenance of Undifferentiated Spermatogonia in Mouse Male Germ Cells
Figure 4
Expression of VRK1 in undifferentiated spermatogonia.
(A, B) Immunocytochemistry of spermatogonia sorted by Ep-CAM antibody with VRK1 and Oct-4 (A) or Plzf (B). (C, D) Spermatogonia were stained with VRK1 and c-kit, and spermatogonia expressing VRK1 were negatively stained with c-kit (C). Heterogeneous populations stained with VRK1 were shown in c-kit-positive spermatogonia (D). (E) Flow cytometry analysis of VRK1-expressing spermatogonia that were then gated by c-kit− and α6-integrin+ in the isolated Ep-CAM+ fraction of heterozygous spermatogonia. Cells were freshly stained with FDG.