LMNA E82K Mutation Activates FAS and Mitochondrial Pathways of Apoptosis in Heart Tissue Specific Transgenic Mice
Figure 1
Generation of the transgenic mice.
(A) The LMNA E82K transgenic construct was generated by inserting the target genes under the control of the α-MHC heart tissue specific promoter and the transgenic mice were created following microinjection. (B) Screening of mouse genomic DNA by PCR for the presence of LMNA E82K gene. M: molecular weight marker. Lane 1: positive control of LmnaE82K transgenic mice; lane 2: negative control; lane 3: blank control; lane 5 and 6: positive LmnaE82K transgenic mice; lane 4 and 7: negative transgenic mice. (C) The mouse lines, founder 30 and 35, with over-expression of LMNA E82K were selected by the western blot procedure using GAPDH as normalization.