Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Determinants of Initiation Codon Selection during Translation in Mammalian Cells

Figure 4

Effect of LNA-AUG1 is independent of different 5′ leader lengths.

A. CAT-FLAG protein expression from AUG1 normalized with that from AUG2 in (CAA)n CAT-FLAG mRNAs. COS-7 cells were transfected with plasmid constructs that express (CAA)4, (CAA)10, or (CAA)16 CAT-FLAG mRNAs, along with a control plasmid that expresses the control FLAG-Luc2 mRNA. LNA oligonucleotides (100 nM) were cotransfected into COS-7 cells as indicated. Expression levels of the two CAT-FLAG proteins were analyzed as in Figure 2. B. CAT-FLAG protein expression from AUG1 normalized with that from AUG2 in COS-7 cells transfected with a plasmid construct that expresses 0.25X, 0.5X, or 1.0X β-globin CAT-FLAG mRNA. At least three independent experiments were performed for final quantification of the immunoblot with error bars indicating standard deviations. For these experiments, cotransfection of cells with LNA-AUG1 resulted in significant differences in relative AUG codon usage (one-sided t-test: * p<0.05; ** p<0.01); no significant change in relative AUG codon usage was observed in cells cotransfected with LNA-C (p>0.05).

Figure 4

doi: https://doi.org/10.1371/journal.pone.0015057.g004