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Proteasome Inhibition Represses Unfolded Protein Response and Nox4, Sensitizing Vascular Cells to Endoplasmic Reticulum Stress-Induced Death

Figure 2

Activity of 20S proteasome in VSMC at control condition or after incubation with AngII (200 nM) or Tn (5 µg/mL), in the absence or presence of MG132 (1 µM) during 4 (A) or 16 (B) hours.

Cell lysates were incubated with probe AMC (LLVY-AMC) in the presence of SDS and fluorescence release followed over 15–30 min (excitation 355 nm, emission 460 nm). Data are mean ± SD of 4 or more independent experiments.*P<0.05 vs. Control. #P<0.05 vs. Tn.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0014591.g002