Short-term Preservation of Porcine Oocytes in Ambient Temperature: Novel Approaches
Figure 4
Effects of different media on mitochondria distribution in porcine oocytes after preservation.
A. a, peripheral mitochondrial distribution, with no distribution at the center of the oocytes; b, semi-peripheral mitochondrial distribution; c, mitochondrial relocation and uniform distribution in the inner region of oocyte cytoplasm. Red, Mito Tracker Red staining. Scale bar = 10 µm. B. Ratios of different mitochondria distribution patterns within the porcine oocytes after preservation with different media. Porcine oocytes were preserved in TCM-199, pFF and FCS at 27.5°C for 3 d, and then mitochondria distribution was stained with MitoTracker Red CMXRos. Three categories of mitochondria distribution were detected: peripheral; semi-peripheral; diffuse. The graph shows the mean ± SEM of three independent experiments. The number “n” in the bracket means the total treated oocyte in every group. The superscripts a, b, c over the bars represent values that differ significantly in every categories of mitochondrial distribution (P<0.05).