De Novo Transcriptome Sequencing in Anopheles funestus Using Illumina RNA-Seq Technology
Figure 4
Variation in transcript divergence among immune gene functional classes.
Protein sequence divergence was estimated as the proportion of aligned amino acids that differ between 1∶1 An. funestus:An. gambiae orthologues. As a class, immune gene orthologous pairs (dotted line indicates mean divergence between immune gene orthologues) are significantly more diverged than the transcriptome as a whole (solid line indicates mean divergence across the entire transcriptome; p-value = 4.8×10−5, Mann-Whitney U-test). The functional classes within the immune genes are not significantly different from each (p-values>0.05, pairwise Mann-Whitney U-tests).