IFN-γ Upregulates Survivin and Ifi202 Expression to Induce Survival and Proliferation of Tumor-Specific T Cells
Figure 9
Silencing ifi202 expression decreased T cell proliferation.
A. Silencing of ifi202 expression by ifi202-specific shRNA. 5KC cells were transduced with scramble shRNA-expressing lentiviral particles (5KC.scramble) and ifi202-specific shRNA (5KC.ifi202.shRNA), respectively. The transduced cells were selected with puromycin for stable lines and analyzed for ifi202 expression by RT-PCR. B. Silencing ifi202 expression decreased T cell proliferation. 5KC.scramble and 5KC.ifi202.shRNA cells were seeded in 96-well plates at the indicated density in the presence of IFN-γ (100 U/ml) for approximately 48 h and analyzed by MTT assay. ** p<0.01. C. Cells were seeded in a 96-well plate as described in B at a density of 3×103 cells/well and measured for 3H incorporation. ** p<0.01.