Silencing of PTK7 in Colon Cancer Cells: Caspase-10-Dependent Apoptosis via Mitochondrial Pathway
Figure 1
PTK7 expression in HCT 116 cells after treatment with vehicle, nonspecific siRNA and PTK7 siRNA.
(A) Flow cytometry assay for the binding of the PE-labeled anti-PTK7 with HCT 116 cells (Grey curves). The black curves represent the background binding of anti-IgG-PE. The concentration of the antibody in the binding buffer was 2 µg/µL. (B) Western blot analysis of PTK7 in HCT 116 cells transfected by PTK7 siRNAs. The membrane was stripped and reprobed by β-actin antibody as a loading control. (C) Suppression of PTK7 mRNA expression in HCT 116 cells by PTK7 siRNAs. Cells were harvested after 48 h of treatment. RT-PCR was performed using gene-specific primers. The amount of PTK7 mRNA expression was normalized to the untreated group. Data are mean±s.d. of three independent experiments. *Student's t-test: P<0.05.