The Human Host Defense Peptide LL-37 Interacts with Neisseria meningitidis Capsular Polysaccharides and Inhibits Inflammatory Mediators Release
Figure 8
Electrophoretic mobility gel shift assay of meningococcal CPS-lpxA polymers in the presence of LL-37, LBP and CD14.
CPS-lpxA complexes were prepared as described in the Methods section, were run on 8% native PAGE, and detected by Western blot using biotinylated LBP antibody. Lane 1 (MWt): molecular weight marker; lane 2 CPS-lpxA alone; lane 3: CPS-lpxA-CD14-LBP; lane 4: CD14-LBP alone; lane 5: CPS-lpxA-LL-37-CD14-LBP; lane 6: LL-37-CD14-LBP; lane 7: CPS-lpxA-LL-37 alone.