Basic Fibroblast Growth Factor Activates MEK/ERK Cell Signaling Pathway and Stimulates the Proliferation of Chicken Primordial Germ Cells
Figure 4
bFGF signaling in chicken PGCs.
(A) Activation of MAPK and PI3K/AKT signaling pathways by bFGF (10 ng·ml−1). NT: no treatment (B) Dose-dependent activation of MEK/ERK signaling by bFGF. NT: no treatment (C) Temporal effect of bFGF (10 ng·ml−1) on activation of MEK/ERK signaling. NT: no treatment (D) Inhibition of MEK/ERK signaling by a specific inhibitor of FGFRs (PD173074). NT: no treatment (E) Inhibition of ERK activation by PD0325901 in cultured PGCs (PD173074 was used as a control). (F) Cell recovery 4 days after PD0325901 treatment (mean ± SEM, n = 4). bFGF (10 ng·ml−1) was treated in all groups. Superscripts indicate significant differences between treatment groups (p<0.05). Statistical analyses were conducted with ANOVA using SAS software. (G–H) Morphology of PGCs 4 days after treatment with PD0325901 (0.4 µM) (bar = 100 µm). bFGF (10 ng·ml−1) was treated in all groups.