Distinct Role of Rab27a in Granule Movement at the Plasma Membrane and in the Cytosol of NK Cells
Figure 4
High level of F-actin hinders LG movement in the cytosol.
NKL cells were transfected with actin-GFP. Perforin containing LG were stained by anti-perforin monoclonal antibody followed by Alexa Fluor conjugated secondary antibody. The representative images from DMSO vehicle control (A) and 1.0 µM Jasp treatment (B) were shown individually. Scale bar is 2.0 µm. The data are representative of at least three independent experiments.