The Unfolded Protein Response Is Not Necessary for the G1/S Transition, but It Is Required for Chromosome Maintenance in Saccharomyces cerevisiae
Figure 3
ER stress in the absence of UPR signaling decreases the rate of cell size increase, but it does not affect the critical budding size.
Synchronous early-G1 cultures of IRE1+ (strain X2180-5B) and ire1Δ (strain SCMSP176) were obtained by elutriation. Half of the elutriated culture for each strain was exposed to 1 mM DTT. The rate of cell size increase (A), and (C), and the critical budding size (B), and (D), were then monitored for IRE1+, and ire1Δ cells, respectively.