αB Crystallin Is Apically Secreted within Exosomes by Polarized Human Retinal Pigment Epithelium and Provides Neuroprotection to Adjacent Cells
Figure 3
Preferential secretion of αB crystallin into the apical domain of polarized RPE monolayers.
(A) Secretion of αB crystallin from human RPE monolayers (mean TER 386 Ω·cm2) was measured from apical and basolateral medium. (B) Immunofluorescence staining of αB crystallin in RPE monolayers showing predominant apical staining as compared to central and basal regions. (C) Co-localization of αB crystallin (green) with CD63 (red) in polarized RPE cells. Scale bar = 20 µm.