DNA Clasping by Mycobacterial HU: The C-Terminal Region of HupB Mediates Increased Specificity of DNA Binding
Figure 1
HupBMtb shows higher binding affinity to double stranded DNA.
Comparative gel retardation analysis showing the binding of increasing amounts of (A) HupBMtb (0–120 nM, lanes 1–12) and (C) HupBMtbN (0–1200 nM, lanes 1–15) to radioactively labeled ds DNA under low salt conditions (10 mM KCl) (sequences shown in Table 2). Dissociation constants (Kd) were also calculated (B) for HupBMtb and (D) for HupBMtbN binding to ds DNA. The Kd values are indicated as inset in the respective panels. Sequence of the oligonucleotide A1 used here is provided at the bottom of the figure.