α-Synuclein Suppression by Targeted Small Interfering RNA in the Primate Substantia Nigra
Figure 5
Effect of α-synuclein siRNA on α-synuclein protein in the monkey substantia nigra.
α-Synuclein or luciferase siRNA was unilaterally infused into the left substantia nigra. Midbrain sections were immunostained with an antibody against α-synuclein. Representative images from an animal receiving α-synuclein siRNA show more robust α-synuclein immunoreactivity within the neuropil of the right (untreated, A) vs. left (siRNA-infused, B) substantia nigra. Scale bar = 5 µm. (C) Optical density measurements of nigral α-synuclein immunoreactivity. Data are expressed as percent of the control value in the right (untreated) substantia nigra and represent mean ± SEM. A significant decrease is caused by α-synuclein but not luciferase siRNA in the left (siRNA-infused) hemisphere. *p<0.03.