A Comparative Analysis of Extra-Embryonic Endoderm Cell Lines
Figure 5
Heat map representation of detailed marker analysis.
Microarray data of markers distinguishing VE from DE according to Sherwood et al. [2]. Only a subset of the previously examined pan endodermal markers are expressed in the array. This refines the list of true pan endodermal markers to include Sox17, Spink3, Rab15, Dsg2, Ripk4, AnxA4 and Emb. “VE enriched” genes indicate factors that were found to be expressed in VE plus other lineages but not DE. Our analysis suggests that a subset of these factors may not be present in all VE subtypes and thus may represent regionalized VE markers. “DE enriched” represents genes found, in Sherwood et al. [2], to be expressed in DE and other subtypes but not in VE. These data strongly suggest that the ExEn cell lines are not similar to DE. Fluorescence data was marked as 0.00 if the p-value of detection was greater than 0.01.