R5 Clade C SHIV Strains with Tier 1 or 2 Neutralization Sensitivity: Tools to Dissect Env Evolution and to Develop AIDS Vaccines in Primate Models
Figure 1
Selection of a neutralization escape variant in rhesus monkey RPn-8.
This monkey was inoculated initially with an “early” form of the virus (the parental infectious molecular clone, SHIV-1157i; see Table 1), and 4 weeks after RPn-8 had progressed to AIDS (∼2.7 years), the “late” virus, SHIV-1157ipd3N4 [1], was generated by molecular cloning of the 3′ proviral half from RPn-8 PBMC DNA. Neutralization of SHIV-1157ip (“early” strain, open triangles) and SHIV-1157ipd3N4 (“late” strain, closed circles) with autologous plasma from animal RPn-8 at the time points post-inoculation indicated is shown (A–D), including contemporaneous plasma for the “late” virus collected at week 139 (panel C). Virus (grown in RM PBMC) was incubated with different dilutions of monkey plasma for 1 h before being added to TZM-bl cells in the presence of DEAE-dextran. Luciferase expression was measured on day 2 post-infection.