Paradoxical Role of Prion Protein Aggregates in Redox-Iron Induced Toxicity
Figure 5
Autophagosomes increase with prion disease progression.
(A) Immunoblotting of scrapie infected hamster brains with LC3 antibody shows increase in LC3-II levels with disease progression relative to age-matched controls (lanes 1–9 vs. 10–15). (B) Quantitative estimation after normalization with β-actin shows significant increase in LC3-II relative to LC3-I in diseased samples 9 and 12 weeks post-inoculation relative to matched controls. *p<0.001; **p<0.01 as compared to NHa. In addition, diseased samples show a significant increase in LC3-II in 9 and 12 weeks post-inoculation samples relative to the 6 week sample. $p<0.001 relative to ScHa at 6 weeks.