Insertion of Vaccinia Virus C7L Host Range Gene into NYVAC-B Genome Potentiates Immune Responses against HIV-1 Antigens
Figure 8
Long-lived responses to vaccination after ENV stimulation.
Splenocytes obtained 53 days post- immunization from animals vaccinated with DNA-B plus NYVAC-B or NYVAC-B-C7L were used to analyze HIV-1-specific CD4+ and CD8+ T cell functions after stimulation with the same peptide pools used previously. Cells were gated by forward and side scatter, CD3+ T cells and then CD4+ and CD8+ T cells. In the figure only HIV-1 specific CD4+ and CD8+T cells stimulated ex vivo with ENV peptide pool are shown. A) Representative flow cytometry plots. The numbers indicate the percentage of CD4+ or CD8+ T cells expressing cytokine(s) IFN-γ and/or IL-2. B) Percentages of CD4+ and CD8+ T cells secreting IFN-γ and/or IL-2 in each immunization group after stimulation with HIV-1 ENV pool.