Impaired Cell Surface Expression of HLA-B Antigens on Mesenchymal Stem Cells and Muscle Cell Progenitors
Figure 5
Kinetics of HLA class I gene transcripts upon IFNγ stimulation and correlation between allele-specific surface protein expression and mRNA expression.
(A) Quantitative measurements of constitutive and up-regulated allele-specific HLA class I gene transcripts pre- and post-IFNγ stimulation by quantitative RT-PCR for DD8 cells. (B) Correlation between constitutive and up-regulated expression of HLA-A and -B allelic gene transcripts and the capacity of antibody binding to the surface HLA class I allelic proteins by indirect flow cytometry staining for HLA-A2, -B7 and -B27. HLA-B*07 showed the lowest constitutive mRNA expression while HLA-B27 showed the lowest constitutive protein expression.