Prevention of Diabetes in NOD Mice by Repeated Exposures to a Contact Allergen Inducing a Sub-Clinical Dermatitis
Figure 2
Topical exposure stimulates NKT cells in A-LN's.
The figures show the number of NKT cells in the A-LN. The A-LN cells were gated to exclude nonviable cells and sample data were collected on 300,000 cells. It can be seen that both allergens stimulates NKT cells in the A-LN though PPD appear to be a stronger or longer stimulator. The increase in cells are followed by a steep drop that may be due to down regulation of the T cell receptor which have been reported previously [35]. Panel A and B shows different experiments. A Mann-Whitney U test was conducted on the experiment in panel A while panel B is included to show other experiments. The Mann-Whitney U tests performed on the experiment in panel A was performed on both the quantity of NKT in relation to the control groups (0 h) and on the number of NKT cells. For the quantity of NKT cells the Mann-Whitney U test was significant (z = −2.095, P<0.05) while the result for the number of NKT cells was insignificant (z = −1.567, P>0.05).