MAPk Activation Modulates Permeability of Isolated Rat Alveolar Epithelial Cell Monolayers Following Cyclic Stretch
Figure 2
SP600125 inhibits JNK phosphorylation.
Phosphorylation levels of JNK, ERK, and p38 MAPk following treatment with the inhibitor SP600125 (20 or 35 µM) or the control DMSO and 10 minutes of cyclic stretch at a rate of 0.25 Hz to a peak magnitude of 37% ΔSA normalized to stretched DMSO controls. Phosphorylation levels of all MAPks significantly increased above unstretched levels in DMSO treated wells. Both concentrations of SP600125 eliminated significant increases in JNK above unstretched levels. Stretch with a treatment of 35 µM of SP600125 resulted in significantly increased ERK phosphorylation compared to DMSO treated stretched wells. Significance is defined as p<0.05, and is depicted with (—) compared to unstretched, and (δ, lower) (*, higher) compared to DMSO treated. Representative western blots of phosphorylated and total MAPks with each treatment are also presented.