Different Functions of the Insect Soluble and Membrane-Bound Trehalase Genes in Chitin Biosynthesis Revealed by RNA Interference
Figure 3
Immunocytochemical analysis of SeTre-1 and SeTre-2 in various tissues.
Various tissues were dissected on day two of the fifith instar larvae and incubated with anti-SeTre-1 or 2 sera followed by goat anti-rabbit IgG conjugated with DAB. Cuticle (A), midgut (B), Malpighian tubules (C), tracheae (D) and fat body (E) were stained (red arrow heads indicate the positive staining). Tissues immunostained with anti-SeTre-1 (Ai-Ei) serum and anti-SeTre-2 (Aii-Eii) serum were stained respectively. Cryosections and tissues immunostained with preimmune serum were used as negative controls (Aiii-Eiii).