Nitrated α-Synuclein Induces the Loss of Dopaminergic Neurons in the Substantia Nigra of Rats
Figure 4
N-SYN induces apoptosis of SHSY-5Y cells.
Apoptosis was determined by TUNEL staining after incubating SHSY-5Y cells with the indicated concentration of N-SYN, SYN or N-GFP for 5 h. Representative images of cells in each treatment group are shown in (A). Typical apoptotic cells (marked by asterisks) displayed chromatin condensation accompanied by shrinking of the soma (Hoechst, blue; TUNEL, red). (B) Quantification of the proportion of apoptotic cells in each treatment group. Data are expressed as percent of TUNEL-positive vs. Hoechst-positive cells and presented as mean ± SEM from at least three independent experiments. *, p<0.05; **, p<0.01. Scale bar, 40 µm.