Subinhibitory Concentrations of Thymol Reduce Enterotoxins A and B and α-Hemolysin Production in Staphylococcus aureus Isolates
Figure 2
Western blot analysis of α-hemolysin, SEA and SEB production.
Both strain ATCC 29213 (A) and MRSA 2985 (B) were cultured with graded subinhibitory concentrations of thymol to an OD600 of 2.5. Supernatants were subjected to SDS-PAGE. After transfer to polyvinylidene fluoride membranes, proteins were stained with the indicated antibodies against α-hemolysin, SEA and SEB. Horseradish peroxidase-conjugated goat anti-rabbit antiserum was used as secondary antibody, and the blots were developed using the ECL substrate (GE Healthcare, UK).