Mass Spectrometric Analysis of Ehrlichia chaffeensis Tandem Repeat Proteins Reveals Evidence of Phosphorylation and Absence of Glycosylation
Figure 3
Immunoprecipitation of TRP47 with anti-pTyr antibody.
Whole cell lysates from uninfected (THP-1) and E. chaffeensis-infected THP-1 cells (ECH) probed with anti-TRP47 antibody [lanes 1 and 2]. ECH whole cell lysates immunoprecipitated with, mouse anti-pTyr antibody (pTyr-IP, lane 3), normal mouse IgG (IgG-IP, lane 4) and detected with TRP47 antibody.