Flagellin-Induced Corneal Antimicrobial Peptide Production and Wound Repair Involve a Novel NF-κB–Independent and EGFR-Dependent Pathway
Figure 5
Mutation in TLR5 binding region inhibits flagellin- or live PAK-induced IL-6, IL-8, but not hBD2 and LL-37, production in HCECs.
HUCL cells were challenged with either 250 ng/ml flagellin (A–C) purified wild-type (PF) PAK, PAK/L94A (LF), and PAK/Q83A (QF) or live bacteria (D-F) for 4 hr. Culture supernatants were analyzed for IL-6, IL-8 by ELISA (A and D) or for hBD2 and LL-37 production using slot-blot (B and E). The intensity of each dots was determined by Photoshop histogram analysis and expressed mean of pixels (C and F). Data shown are representative of two independent experiments. *P<0.01 (N = 3).