The Site Specific Demethylation in the 5′-Regulatory Area of NMDA Receptor 2B Subunit Gene Associated with CIE-Induced Up-Regulation of Transcription
Figure 3
CIE induced DNA demethylation in the specific CpG sites determined by bisulfite-pyrosequencing.
This graph outlines the relative CpG positions, where CIE induced significantly demethylation, to the transcription start site and the binding sites of AP-1 and CREB. Methylation status of individual CpG was determined by bisulfite-pyrosequencing at 116 CpG sites in the 5′-regulatory area of the NR2B gene. All bars represent the average basal methylation level (%) of individual CpG (control cells). The arrows in red only indicate these CpG sites where CIE induced significant demethylation (the detailed alterations are shown in Figure 4). The locations of transcription factor AP-1 and CREB binding sites are indicated by arrow. The bent arrow indicates the location of transcription start site.