Localisation and Mislocalisation of the Interferon-Inducible Immunity-Related GTPase, Irgm1 (LRG-47) in Mouse Cells
Figure 6
Synthetic αK amphipathic peptide mimics the localisation of endogenous Irgm1.
MEFs were treated with 200 U/ml IFNγ for 24 hours and then fixed with 3% PFA in PBS. Cy3-streptavidin-peptide solutions (0.33 µM streptavidin) were prepared as described in Materials and Methods. Antibodies against the indicated proteins were added to the streptavidin-peptide solution and the mixtures used as primary reagents in immunofluorescence staining. Streptavidin (0.33 µM final) alone (a) was used as control. Image (a) and (b) were taken at the same exposure time. Colocalisation was seen between streptavidin-Irgm1 αK peptide and endogenous Irgm1 at both Golgi apparatus and lysosomes.