Co-Inhibition of BCL-W and BCL2 Restores Antiestrogen Sensitivity through BECN1 and Promotes an Autophagy-Associated Necrosis
Figure 6
BECN1 knockdown and BCL-W/BCL2 co-inhibition decreases cell proliferation through increased apoptosis in resistant cells.
(A) Whole cell lysates were subjected to Western blot analysis with a specific BECN1 monoclonal antibody. Bars represent the mean±SE of the relative BECN1∶actin ratio (normalized to control cells) for three independent experiments. (B) shRNA infected MCF-7/LCC9 cells were treated with YC137 and/or ICI for 7-days. Bars represent the mean±SE of relative cell proliferation (normalized to EtOH treated controls) for a single representative experiment performed in triplicate. (C) shRNA infected MCF-7/LCC9 cells were treated with YC137, ICI, or a combination of YC137 and ICI. Bars represent the mean±SE of the relative Annexin V staining (normalized to empty vector controls) for three independent experiments. (D) Bars represent the mean±SE of the relative PI staining (normalized to empty vector controls) for three independent experiments.