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NPR1 Protein Regulates Pathogenic and Symbiotic Interactions between Rhizobium and Legumes and Non-Legumes

Figure 3

Gene expression analysis of early and late M. truncatula nodulin gene orthologs in A. thaliana roots.

(A) Roots of ten days old wild-type or nim1/npr1 seedlings grown on nitrogen poor medium were inoculated in zone 1 with either S. meliloti or treated with nod factor (NF). The expression of Arabidopsis ENOD20 homolog (AtENOD20, At5g57920) was tested two days after inoculation, using quantitative Real-time RT-PCR. The results show the mean of 3 independent repeats for each treatment. Error bars represent SD. (B) Roots of ten days old wild-type and nim1/npr1 seedlings grown on nitrogen poor medium were inoculated with S. meliloti or with P.putida. Late nodulin, (AtMtN21, At5g07050) was assayed after 7 days. Plant roots were frozen in liquid nitrogen and gene expression was analyzed by semiquantitative RT-PCR. The RNA samples were normalized according to actin-2 gene expression. Experiments were repeated 3 times with very similar results.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0008399.g003