The Danger Signal Adenosine Induces Persistence of Chlamydial Infection through Stimulation of A2b Receptors
Figure 2
Repeated exposure to cell permeable cAMP analog or cAMP elevating agents causes a reduction in chlamydial development.
HeLa cells were infected with C. trachomatis serovar L2 at an MOI of 1, followed by treatment with adenosine (100 µM), forskolin (FSK, 10 µM), or 8-Br-cAMP (100 µM) starting at 1 hour post infection (hpi) every half-hour through 6 hpi (10 additions). Total RNA was harvested at 24 hpi for quantification of chlamydial 16s rRNA production using qPCR as indicated in methods. The values shown are relative to control values for each experiment, and are averages and S.D. from 3 independent experiments. (n = 3, **, P<0.0005, ***, P<0.0001 for treated cells compared to untreated control.)