Immunological Memory Transferred with CD4 T Cells Specific for Tuberculosis Antigens Ag85B-TB10.4: Persisting Antigen Enhances Protection
Figure 3
CD4 T cells specific for BCG or for Ag85B-TB10.4 transfer protective immunity.
(A) CD45RBlow CD4 T cells were purified from donors primed and boosted with BCG (Pr/Boost) and injected (3×106 or 0.3×106) into irradiated recipients (B, C). Control recipients received 3×106 unprimed (Naïve) CD4 T cells or no cells (None). (D) CD45RBlow CD4 T cells were purified from donors primed with Ag85B-TB10.4 and boosted with Ag85B-TB10.4 and 3×106 or 0.3×106 T cells injected into irradiated recipients. Control recipients received 3×106 unprimed (Naïve) CD4 T cells or no cells (None). All recipients in experiments A and D were challenged i.v. with BCG the day after transfer, killed 3 weeks later and assayed for BCG CFU/spleen (B, E). Each point represents a single recipient. Donor-derived splenic CD4 T cells stimulated in vitro with Ag85B-TB10.4 were stained for intracellular cytokine (ICC) IFN-γ (C, F). The results were from single experiments. Horizontal bars or histograms + SD are means of 4 recipients/group. * p<0.05; ** p<0.01.