The ASH1 HOMOLOG 2 (ASHH2) Histone H3 Methyltransferase Is Required for Ovule and Anther Development in Arabidopsis
Figure 6
Changes in gene expression and histone tail methylation in the ashh2-1 mutant.
(A) Functional GO annotation of genes differentially expressed in ashh2-1 inflorescences relative to wt. The inserts depict the developmental stage of the main inflorescences used in the microarray experiment. (B) Number of co-down-regulated genes in the ashh2-1 mutant (>1.6-fold change), ms1 mutant (>2-fold change) and spl/ems-1 mutant (>1.6-fold change). (C) Confirmation of down-regulation of selected genes using real-time reverse transcriptase PCR. Wt levels were set to 1. (D) Gene structures of AtDMC1, AP1 and MYB99. Boxes indicate exons, thin lines introns. The positions of the fragments tested in the ChIP analyses are shown as red lines. (E) Representative results of ChIP analyses on the selected down-regulated genes shown in (D) using antibodies against H3K4me3, H3K36me2 and H3K4me3. Ta3 was used as a control. IN – input; –ab – without antibodies.