SMF-1, SMF-2 and SMF-3 DMT1 Orthologues Regulate and Are Regulated Differentially by Manganese Levels in C. elegans
Figure 9
Genotype and Mn exposure influence on smf gene expression.
Both independent primer sets corresponding to the 3′ end of the cDNA and used to assess smf gene expression (set A bright colors, and set B faded colors) give consistent results (A, B, C). smf-1 and smf-3 mRNA levels display similar variations, increasing at low Mn exposure (0.1 mM) and decreasing upon high Mn concentrations (10 mM and 100 mM) in WT, smf-1 and smf-3 mutants (A, C, black, blue, orange). smf-2 mRNA levels follow the same tendency in smf-1 and smf-3 mutants but are not affected by Mn exposure in WT (B). Independent of Mn exposure, smf-1(eh5) mutant is characterized by a strong up-regulation of smf-2 expression (B, blue). Compared to other genotypes, smf expression in smf-2(gk133) does not appear to correlate with Mn exposure (A, B, C, green). The smf-3(ok1035) mutant exhibits higher smf gene expression levels regardless of Mn exposure dose (A, B, C, orange). While # designate significant differences between genetic backgrounds exposed to the same manganese dose, * indicate significant differences between exposure doses within the same C. elegans strain: #/* p<0.05. ##/** p<0.01, ###/p<0.001. Displayed significance levels between treatments reflect the weakest significance score obtained between both primer sets.