Expression of Telomerase and Telomere Length Are Unaffected by either Age or Limb Regeneration in Danio rerio
Figure 3
Telomerase activity is present in the organs of aged fish.
Pooled organs from 3 fish per age group were dissected and flash frozen in liquid nitrogen until used. Protein was extracted using CHAPS buffer and 1 ug of total protein used in a TRAP assay. Results are shown as OD (450–690) above background (Rnase treated lysates). All samples performed in duplicate. WKM denotes whole kidney marrow.